| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Huang, Xirui (8)
|
VAODAC |
46.45 |
44.28 |
-2.17 |
| 2 |
Du, Rebecca R (8)
|
VACGBD |
47.14 |
45.25 |
-1.89 |
| 3 |
Scibelli, Stella J (7)
|
VAECAT |
49.32 |
48.17 |
-1.15 |
| 4 |
Ban Boca, Maya E (8)
|
VAWAC |
55.51 |
52.78 |
-2.73 |
| 5 |
Vasquez, Evangeline A (8)
|
VATIDE |
|
54.03 |
- |
| 6 |
Trover, Aeralyn L (8)
|
VANSD |
|
55.63 |
- |
| 7 |
Boyette, Aria C (7)
|
VAODAC |
|
56.72 |
- |
| 8 |
Durrett, Taylor L (8)
|
VAODAC |
|
56.76 |
- |
| 9 |
Pegram, Allison M (8)
|
VACGBD |
55.93 |
57.43 |
1.50 |
| 10 |
Gurnee, Charlotte C (7)
|
VAODAC |
1:03.66 |
57.80 |
-5.86 |
| 11 |
Swartzel, Reagan J (8)
|
VAWAC |
|
57.92 |
- |
| 12 |
Smith, Leilani K (8)
|
VAODAC |
1:07.87 |
58.47 |
-9.40 |
| 13 |
Mikels, Kinsley R (8)
|
VAWAC |
1:01.40 |
58.60 |
-2.80 |
| 14 |
Hamilton, Adeline J (7)
|
VATIDE |
|
58.71 |
- |
| 15 |
Moore, Clara W (7)
|
VACGBD |
59.99 |
58.97 |
-1.02 |
| 16 |
Williams, Sophia J (8)
|
VATIDE |
|
1:02.17 |
- |
| 17 |
Wilhite, Lilliana D (8)
|
VATIDE |
|
1:02.23 |
- |
| 18 |
Kirkpatrick, Lydia B (8)
|
VATIDE |
|
1:03.30 |
- |
| 19 |
Blythe, Victoria A (8)
|
VATIDE |
|
1:04.00 |
- |
| 20 |
Wickre, Alexis J (7)
|
VATIDE |
|
1:04.14 |
- |
| 21 |
Cox, Chloe E (8)
|
VAODAC |
1:05.60 |
1:05.53 |
-0.07 |
| 22 |
Lewis, Penelope H (7)
|
VATIDE |
|
1:05.99 |
- |
| 23 |
Thomas, Ireland I (8)
|
VANSD |
|
1:07.08 |
- |
| 24 |
Osborne, Katerina L (7)
|
VAODAC |
|
1:09.17 |
- |
| 25 |
Carreon, Sophia R (7)
|
VANSD |
|
1:12.70 |
- |
| 26 |
Chapman, Noelle M (7)
|
VATIDE |
|
1:12.98 |
- |
|
Daniels, Lucy C (7)
|
VAODAC |
|
DQ |
- |
|
Vallandingham, Addison L (7)
|
VATIDE |
59.57 |
DQ |
- |
|
Johnson, Audrey G (8)
|
VAECAT |
1:01.59 |
NS |
- |